In Silico Modelling and Metabolic Engineering of Escherichia Coli to Succinic Acid Production

Succinic acid is identified to have a great economical potential in a biobased economy. To improve the production in Escherichia coli metabolic engineering should be carried out. For new mutant strain design is important to in silico simulate and analyze the metabolic changes, network of a cell under different environmental and/or genetic perturbations. To investigate the genetic and environmental perturbations, the relationship between biomass and succinate yield, the NADH oxidation connection to growth rate and succinate production, in silico metabolic analysis was carried out using constraint-based metabolic flux simulations in minimal medium with glucose and glycerol as carbon sources.